Making progress in developing probiotic beverages without animal protein

Most products enriched with probiotics and protein are made with dairy products, however, INRS said consumer demand for plant protein is growing. The nutritional value of these proteins must therefore be improved to be comparable to that of animal proteins.

The team, led by Professor Monique Lacroix, demonstrated fermenting drinks fortified with pea and rice proteins yields the same quality of protein as casein, an animal protein found in milk. The findings were published in the Journal of Food Science​.

Using plant proteins poses some challenges. For one thing, plant proteins are deficient in some essential amino acids. Since the body doesn't produce these amino acids, they must come from food. The combination of pea and rice proteins helped counterbalance essential amino acid deficiencies through complementarity.

The researchers said plant proteins are also harder to digest.

"They are often non-soluble in water and under globular form. That means our digestive enzymes have more difficulty breaking them down. Animal proteins, on the other hand, usually take the form of elongated fibers that are easily processed by digestive enzymes,"​ said Professor Lacroix.

But when pea and rice proteins are added before the fermentation stage during production of a fermented beverage, they can be predigested by lactic acid bacteria (LAB). This allows the production of peptides (protein fragments) resulting from the breakdown of proteins during fermentation, thereby facilitating their absorption during digestion.

The study, carried out in collaboration with Bio-K+, used a specific formulation containing the bacterium Lactobacillus acidophilus​ CL1285, L. casei​ LBC80R and L. rhamnosus​ CLR2. These LAB, whose probiotic functions have been scientifically proven and licensed by Health Canada, were used in the study to produce the fermented drink enriched with pea and rice proteins.

The article "Protein quality of a probiotic beverage enriched with pea and rice protein", by Johanne Manus, Mathieu Millette, Chaima Dridi, Stéphane Salmieri, Blanca R. Aguilar Uscanga and Monique Lacroix, was published in the Journal of Food Science.

The study received financial support from the Ministère de l'Économie et de l'Innovation and the Natural Sciences and Engineering Research Council of Canada.